Journal of Medical Virology
Volume 64, Issue 3, 2001, Pages 305-311

Hepatitis B virus genotypes and HBsAg subtypes in refugees and injection drug users in the United States determined by LiPA and monoclonal EIA (Article)

Swenson P.D. , Van Geyt C. , Russell Alexander E. , Hagan H. , Freitag-Koontz J.M. , Wilson S. , Norder H. , Magnius L.O. , Stuyver L.
  • a Seattle/King County Pub. Hlth. Lab., 325 9th Ave., Seattle, WA 98104-2499, United States
  • b Seattle/King County Pub. Hlth. Lab., 325 9th Ave., Seattle, WA 98104-2499, United States
  • c Seattle/King County Pub. Hlth. Lab., 325 9th Ave., Seattle, WA 98104-2499, United States
  • d Seattle/King County Pub. Hlth. Lab., 325 9th Ave., Seattle, WA 98104-2499, United States
  • e Seattle/King County Pub. Hlth. Lab., 325 9th Ave., Seattle, WA 98104-2499, United States
  • f Seattle/King County Pub. Hlth. Lab., 325 9th Ave., Seattle, WA 98104-2499, United States
  • g Seattle/King County Pub. Hlth. Lab., 325 9th Ave., Seattle, WA 98104-2499, United States
  • h Seattle/King County Pub. Hlth. Lab., 325 9th Ave., Seattle, WA 98104-2499, United States
  • i Seattle/King County Pub. Hlth. Lab., 325 9th Ave., Seattle, WA 98104-2499, United States

Abstract

Hepatitis B virus (HBV) genotyping and hepatitis B surface antigen (HBsAg) subtyping were carried out on sera from 196 HBsAg-positive patients, including 151 refugees entering the United States and 45 injection drug users in Seattle. HBsAg subtyping was performed by enzyme immunoassay (EIA) using a panel of monoclonal antibodies and the HBV genotype was determined by polymerase chain reaction (PCR) followed by detection of amplified HBV DNA by a reverse-phase hybridization line probe assay (LiPA) using genotype-specific probes. HBV DNA was detected by PCR in 155 (79%) of the 196 sera and all 155 were genotyped by LiPA. Samples from Southeast Asia were predominantly genotype B/subtype ayw1 and genotype C/adr; samples from the former Soviet Union and eastern Europe were mostly genotype D/ayw2 and genotype D/ayw3; samples from east Africa were mainly genotype A/adw2 and genotype D/ayw2; and samples from injection drug users were mostly genotype D/ayw3 and genotype A/adw2. Some strains of ayw3 gave atypical monoclonal antibody reactivity patterns in the subtyping assay due to a Val/Ala instead of a Thr at amino acid residue 118 and a Thr instead of a Met at residue 125. A strain of ayw2 also gave an atypical monoclonal antibody reactivity pattern due to an Ala instead of a Thr at amino acid residue 123. LiPA genotyping and monoclonal EIA subtyping can provide useful information for epidemiological studies. © 2001 Wiley-Liss, Inc.

Author Keywords

Hepatitis B surface antigen Subtypes Genotypes Monoclonal antibody Line probe assay Hepatitis B virus

Index Keywords

genotype Europe, Eastern refugee virus DNA human Base Sequence Refugees middle aged Middle East USSR Eastern Europe nonhuman Hepatitis B virus nucleotide sequence Haiti United States Consensus Sequence Humans reverse phase hybridization line probe assay monoclonal antibody Antibodies, Monoclonal Southeast Asia Amino Acid Sequence Hepatitis B e Antigens polymerase chain reaction Article Substance Abuse, Intravenous major clinical study assay Northwestern United States gene sequence hepatitis B surface antigen Immunoenzyme Techniques Hepatitis B Surface Antigens hepatitis B Reagent Kits, Diagnostic enzyme immunoassay intravenous drug abuse Asia, Southeastern Africa, Eastern
Link
https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034975293&doi=10.1002%2fjmv.1051&partnerID=40&md5=3c37d5ebac89b40ecc209f0468b4879a
DOI: 10.1002/jmv.1051
ISSN: 01466615
Cited by: 38
Original Language: English