World Allergy Organization Journal
Volume 12, Issue 8, 2019

Western oropharyngeal and gut microbial profiles are associated with allergic conditions in Chinese immigrant children (Article) (Open Access)

Guo J. , Lv Q. , Ariff A. , Zhang X. , Peacock C.S. , Song Y. , Wen X. , Saiganesh A. , Melton P.E. , Dykes G.A. , Moses E.K. , LE Souëf P.N. , Lu F.* , Zhang G.
  • a School of Public Health, Curtin University of Technology, Perth, WA, Australia, Centre for Genetic Origins of Health and Disease, Faculty of Health Sciences, Curtin University of Technology, Faculty of Health and Medical Sciences, The University of Western Australia, Royal Perth Hospital Medical Research Foundation, Perth, WA, Australia
  • b Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou, Henan, China
  • c Centre for Genetic Origins of Health and Disease, Faculty of Health Sciences, Curtin University of Technology, Faculty of Health and Medical Sciences, The University of Western Australia, Royal Perth Hospital Medical Research Foundation, Perth, WA, Australia
  • d China National Bamboo Research Centre, Key Laboratory of Resources and Utilization of Bamboo of State Forestry Administration, Hangzhou, Zhejiang, China
  • e Marshall Centre for Infectious Disease, School of Pathology and Laboratory Medicine, The University of Western Australia, Perth, WA, Australia, Telethon Kids Institute, The University of Western Australia, Perth, WA, Australia
  • f School of Public Health, Curtin University of Technology, Perth, WA, Australia, Centre for Genetic Origins of Health and Disease, Faculty of Health Sciences, Curtin University of Technology, Faculty of Health and Medical Sciences, The University of Western Australia, Royal Perth Hospital Medical Research Foundation, Perth, WA, Australia
  • g Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou, Henan, China
  • h Telethon Kids Institute, The University of Western Australia, Perth, WA, Australia, Division of Cardiovascular and Respiratory Sciences, The University of Western Australia, Perth, WA, Australia
  • i Centre for Genetic Origins of Health and Disease, Faculty of Health Sciences, Curtin University of Technology, Faculty of Health and Medical Sciences, The University of Western Australia, Royal Perth Hospital Medical Research Foundation, Perth, WA, Australia, School of Pharmacy and Biomedical Sciences, Faculty of Health Sciences, Curtin University of Technology, Perth, WA, Australia
  • j School of Public Health, Curtin University of Technology, Perth, WA, Australia
  • k Centre for Genetic Origins of Health and Disease, Faculty of Health Sciences, Curtin University of Technology, Faculty of Health and Medical Sciences, The University of Western Australia, Royal Perth Hospital Medical Research Foundation, Perth, WA, Australia, School of Pharmacy and Biomedical Sciences, Faculty of Health Sciences, Curtin University of Technology, Perth, WA, Australia
  • l Division of Cardiovascular and Respiratory Sciences, The University of Western Australia, Perth, WA, Australia
  • m Department of Microbiology & Infectious Disease Centre, School of Basic Medical Sciences, Peking University Health Science Centre, Beijing, China
  • n School of Public Health, Curtin University of Technology, Perth, WA, Australia, Centre for Genetic Origins of Health and Disease, Faculty of Health Sciences, Curtin University of Technology, Faculty of Health and Medical Sciences, The University of Western Australia, Royal Perth Hospital Medical Research Foundation, Perth, WA, Australia, Telethon Kids Institute, The University of Western Australia, Perth, WA, Australia, Division of Cardiovascular and Respiratory Sciences, The University of Western Australia, Perth, WA, Australia

Abstract

Background: The allergy epidemic resulting from western environment/lifestyles is potentially due to modifications of the human microbiome. Therefore, it is of interest to study immigrants living in a western environment as well as their counterparts in the country of origin to understand differences in their microbiomes and health status. Methods: We investigated 58 Australian Chinese (AC) children from Perth, Western Australia as well as 63 Chinese-born Chinese (CC) children from a city in China. Oropharyngeal (OP) and fecal samples were collected. To assess the microbiomes, 16s ribosomal RNA (rRNA) sequencing for variable regions V3 and V4 was used. Skin prick tests (SPT) were performed to measure the children's atopic status. Information on food allergy and wheezing were acquired from a questionnaire. Results: AC children had more allergic conditions than CC children. The alpha diversity (mean species diversity) of both OP and gut microbiome was lower in AC children compared to CC children for richness estimate (Chao1), while diversity evenness (Shannon index) was higher. The beta diversity (community similarity) displayed a distinct separation of the OP and gut microbiota between AC and CC children. An apparent difference in microbial abundance was observed for many bacteria. In AC children, we sought to establish consistent trends in bacterial relative abundance that are either higher or lower in AC versus CC children and higher or lower in children with allergy versus those without allergy. The majority of OP taxa showed a consistent trend while the majority of fecal taxa showed a contrasting trend. Conclusion: Distinct differences in microbiome compositions were found in both oropharyngeal and fecal samples of AC and CC children. The association of the OP microbiome with allergic condition is different from that of the gut microbiome in AC children. The microbiome profiles are changed by the western environment/lifestyle and are associated with allergies in Chinese immigrant children in Australia. © 2019 The Authors

Author Keywords

Microbiome Immigration Allergy Western environment Atopy

Index Keywords

China immigrant Chinese human trend study Shigella RNA 16S controlled study priority journal nonhuman wheezing cross-sectional study atopy prick test male female intestine flora questionnaire Western Australia population research Article Escherichia oropharyngeal microbiome Ruminococcus Parasutterella Proteobacteria Barnesiella microbiome food allergy Porphyromonadaceae Clostridiales Firmicutes RNA sequence species richness microbial diversity Peptoclostridium Burkholderiales Lachnospira Eubacterium Child
Link
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070319662&doi=10.1016%2fj.waojou.2019.100051&partnerID=40&md5=65b138fb7b1d701bcdc84d625e0e8ea9
DOI: 10.1016/j.waojou.2019.100051
ISSN: 19394551
Original Language: English