Journal of Food Biochemistry
Volume 42, Issue 5, 2018

How do methanol and higher alcohols found in alcoholic beverages affect membrane fluidity and migration of granulocytes? (Article)

Pál L. , Bujdosó O. , Szűcs S.* , Baranyi G. , Sebestyén V. , Vámosi G. , Rácz G. , Ádány R. , McKee M. , Árnyas E.M.
  • a Faculty of Public Health, Department of Preventive Medicine, University of Debrecen, Debrecen, Hungary
  • b Faculty of Public Health, Department of Preventive Medicine, University of Debrecen, Debrecen, Hungary
  • c Faculty of Public Health, Department of Preventive Medicine, University of Debrecen, Debrecen, Hungary
  • d Faculty of Public Health, Department of Preventive Medicine, University of Debrecen, Debrecen, Hungary
  • e Faculty of Medicine, Department of Biophysics and Cell Biology, University of Debrecen, Debrecen, Hungary
  • f Faculty of Medicine, Department of Biophysics and Cell Biology, University of Debrecen, Debrecen, Hungary
  • g Faculty of Public Health, Department of Preventive Medicine, University of Debrecen, Debrecen, Hungary
  • h Faculty of Public Health, Department of Preventive Medicine, University of Debrecen, Debrecen, Hungary, MTA-DE Public Health Research Group of Hungarian Academy of Sciences, University of Debrecen, Debrecen, Hungary
  • i European Centre on Health of Societies in Transition, London School of Hygiene and Tropical Medicine, London, United Kingdom
  • j Faculty of Public Health, Department of Preventive Medicine, University of Debrecen, Debrecen, Hungary

Abstract

Ethanol-induced changes in membrane fluidity and migration of granulocytes has been suggested to contribute to development of alcoholic hepatitis. Besides ethanol other aliphatic alcohols (OAAs) have been detected in alcoholic beverages. We examined the effects of OAAs alone and in combination with ethanol on these processes. Granulocytes isolated from human blood were treated separately with methanol, ethanol, and a mixture of OAAs including methanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, isobutanol, and isoamyl alcohol at biologically relevant concentrations. The membrane fluidity and migration activity of granulocytes was examined by fluorescence anisotropy method and migration chambers, respectively. Our findings showed that OAAs increased membrane fluidity and migration activity of granulocytes in a concentration-dependent manner and when combined with ethanol they caused a further increase in the migration activity of granulocytes. It is possible that OAAs consumed with alcoholic beverages may further enhance migration of granulocytes in heavy drinkers and contribute to ethanol-induced liver damage. Practical applications. The consumption of OAAs in alcoholic beverages has been hypothesized as an additional risk factor for the development of alcoholic liver diseases. To confirm this suggestion, the potentially adverse effects of exposure to OAAs should be further examined. Our investigation provides new toxicological data on the effects of OAAs found in alcoholic beverages. The results of this study can be used for more precise toxicological evaluations to clarify whether OAAs may pose an additional risk factor for the development of alcoholic liver diseases. Furthermore, information on the harmful effects of OAAs could enable health policy makers to make evidence-based decisions when implementing new regulations on alcoholic beverages. © 2018 Wiley Periodicals, Inc.

Author Keywords

Migration membrane fluidity alcoholic liver damage granulocytes aliphatic alcohols alcohol consumption

Index Keywords

Alcoholic Beverages Membrane fluidity Membranes Fluidity granulocytes Aliphatic alcohol Methanol Liver damage alcohol consumption migration Ethanol
Link
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052536330&doi=10.1111%2fjfbc.12593&partnerID=40&md5=7661073deeb37120e17e289dff0a33b2
DOI: 10.1111/jfbc.12593
ISSN: 01458884
Original Language: English