Journal of Clinical Microbiology
Volume 43, Issue 7, 2005, Pages 3460-3462

Improved sensitivity of sputum smear microscopy after processing specimens with C18-carboxypropylbetaine to detect acid-fast bacilli: A study of United States-bound immigrants from Vietnam (Article) (Open Access)

Laserson K.F.* , Yen N.T.N. , Thornton C.G. , Mai V.T.C. , Jones W. , An D.Q. , Phuoc N.H. , Trinh N.A. , Nhung D.T.C. , Lien T.X. , Lan N.T.N. , Wells C. , Binkin N. , Cetron M. , Maloney S.A.
  • a Centers for Disease Control and Prevention, Atlanta, GA, United States, Division of Tuberculosis Elimination, Centers for Disease Control and Prevention, MS E-10, 1600 Clifton Road, Atlanta, GA 30333, United States
  • b Cho Ray Hospital, Ho Chi Minh City, Viet Nam
  • c Integrated Research Technology, LLC, Baltimore, MD, United States
  • d Cho Ray Hospital, Ho Chi Minh City, Viet Nam
  • e Centers for Disease Control and Prevention, Atlanta, GA, United States, International Organization of Migration, Ho Chi Minh City, Viet Nam
  • f Cho Ray Hospital, Ho Chi Minh City, Viet Nam
  • g Cho Ray Hospital, Ho Chi Minh City, Viet Nam
  • h Cho Ray Hospital, Ho Chi Minh City, Viet Nam
  • i Centers for Disease Control and Prevention, Atlanta, GA, United States
  • j Institute Pasteur, Ho Chi Minh City, Viet Nam
  • k Institute Pasteur, Ho Chi Minh City, Viet Nam
  • l Centers for Disease Control and Prevention, Atlanta, GA, United States
  • m Centers for Disease Control and Prevention, Atlanta, GA, United States
  • n Centers for Disease Control and Prevention, Atlanta, GA, United States
  • o Centers for Disease Control and Prevention, Atlanta, GA, United States

Abstract

The goal of this study was to evaluate the effect of the specimen-processing method that uses the detergent C18- carboxypropylbetaine (CB-18) on the sensitivity of acid-fast bacillus (AFB) staining. Vietnamese immigrants with abnormal chest radiographs provided up to three sputum specimens, which were examined for acid-fast bacilli by use of direct auramine and Ziehl-Neelsen staining. The remaining sputum was split; half was cultured, and the other half was incubated with CB-18 for 24 h, centrifuged, and examined for AFB by both staining methods. CB-18 processing improved the sensitivity of AFB staining by 20 to 30% (only differences in auramine sensitivity were statistically significant) but reduced specificity by ≈20% (P < 0.05). These findings have direct utility for overseas migrant tuberculosis screening programs, for which maximizing test sensitivity is a major objective. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Author Keywords

[No Keywords available]

Index Keywords

Vietnam immigrant sputum smear incubation time human bacterium identification Bacilli sputum culture priority journal Mycobacterium tuberculosis sensitivity analysis statistical significance United States Humans Sputum lung tuberculosis Tuberculosis, Pulmonary acid fast bacterium Viet Nam unclassified drug sensitivity and specificity Article major clinical study adult microscopy bacterium detection thorax radiography Specimen Handling Emigration and Immigration c 18 carboxypropylbetaine Betaine Benzophenoneidum betaine derivative detergent Bacillus (bacterium) Staining and Labeling auramine bacterial strain bacterium culture
Link
https://www.scopus.com/inward/record.uri?eid=2-s2.0-22144483094&doi=10.1128%2fJCM.43.7.3460-3462.2005&partnerID=40&md5=c6c4f684a05701b360a5debff65856f4
DOI: 10.1128/JCM.43.7.3460-3462.2005
ISSN: 00951137
Cited by: 17
Original Language: English