International Journal of Tuberculosis and Lung Disease
Volume 14, Issue 8, 2010, Pages 994-1000

A snapshot of the biodiversity and clustering of Mycobacterium tuberculosis in Oman using spoligotyping (Article)

Al-Maniri A.* , Singh J.P.N. , Al-Rawas O. , Al Busaidy S. , Al Balushi L. , Ahmed I. , Al Mahruqi S. , Haile M. , Diwan V.K. , Hoffner S.
  • a Department of Public Health Sciences, Division of Global Health, Karolinska Institute, Stockholm, Sweden, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman
  • b Central Public Health Laboratories, Ministry of Health, Muscat, Oman
  • c College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman
  • d Central Public Health Laboratories, Ministry of Health, Muscat, Oman
  • e Central Public Health Laboratories, Ministry of Health, Muscat, Oman
  • f Central Public Health Laboratories, Ministry of Health, Muscat, Oman
  • g Central Public Health Laboratories, Ministry of Health, Muscat, Oman
  • h Department of Bacteriology, Swedish Institute for Infectious Disease Control, Solna, Sweden, Department of Microbiology, Tumour and Cell Biology, Karolinska Institute, Stockholm, Sweden
  • i Department of Public Health Sciences, Division of Global Health, Karolinska Institute, Stockholm, Sweden
  • j Department of Bacteriology, Swedish Institute for Infectious Disease Control, Solna, Sweden, Department of Microbiology, Tumour and Cell Biology, Karolinska Institute, Stockholm, Sweden

Abstract

SETTINGS: National Tuberculosis Reference Laboratory, Central Public Health Laboratory, Ministry of Health, Oman. OBJECTIVE: To use spoligotyping to explore the genetic population structure and clustering of Mycobacterium tuberculosis isolates among nationals and immigrants in Oman. METHODS: Using spoligotyping, we characterised all available isolates from 2007, and randomly selected isolates from 2005 and 2006. A total of 312 clinical isolates from the same number of patients diagnosed with tuberculosis (TB) in 2005-2007 were included in the study. RESULTS: Of 312 isolates, 69% were in clusters ranging from 2 to 38 isolates. The proportion of clustering was 58% among 2005-2006 samples and 67% among 2007 samples, with higher clustering among Omanis than among immigrants. The study showed that M. tuberculosis Indian family lineages, CAS1-Delhi, CAS and EAI5 were the predominant strains. Around 50% of the immigrants shared strains with Omanis. Twelve of the 19 INH-monoresistant strains and the two multidrug-resistant strains were in clusters (P = 0.81). CONCLUSION: This study demonstrates the predominance in Oman of the strain family commonly found on the Indian sub-continent. A high proportion of immigrant strains were in the same clusters as Omani strains. To better ascertain the transmission dynamics of M. tuberculosis, we recommend that stringent molecular and conventional epidemiological methods be applied. © 2010 The Union.

Author Keywords

immigrants TB elimination Spoligotyping Oman M. Tuberculosis

Index Keywords

genetics genotype immigrant bacterial transmission transmission India Indian human epidemiology middle aged statistics and numerical data multidrug resistant tuberculosis Mycobacterium tuberculosis priority journal comparative study Aged nonhuman ethnology cluster analysis Young Adult Humans migrant Adolescent male Emigrants and Immigrants female tuberculosis Infant prevalence isolation and purification Article Retrospective Studies major clinical study adult bacterial gene spoligotyping isoniazid Biodiversity Oman gene cluster gene structure analytic method (1 butylamino 3 methylbutyl)(1 pyrrolidinyl) ketone microbiology antibiotic resistance bacterium isolate retrospective study bacterial strain Child
Link
https://www.scopus.com/inward/record.uri?eid=2-s2.0-77954737009&partnerID=40&md5=c9daf14a2679892e55b303413f87593b
ISSN: 10273719
Cited by: 7
Original Language: English