Journal of Clinical Endocrinology and Metabolism
Volume 94, Issue 5, 2009, Pages 1781-1788

Fasted to fed trafficking of fatty acids in human adipose tissue reveals a novel regulatory step for enhanced fat storage (Article) (Open Access)

Ruge T. , Hodson L. , Cheeseman J. , Dennis A.L. , Fielding B.A. , Humphreys S.M. , Frayn K.N. , Karpe F.*
  • a Nuffield Department of Clinical Medicine, Oxford Centre for Diabetes, Endocrinology, and Metabolism, University of Oxford, Oxford OX3 7LJ, United Kingdom
  • b Nuffield Department of Clinical Medicine, Oxford Centre for Diabetes, Endocrinology, and Metabolism, University of Oxford, Oxford OX3 7LJ, United Kingdom
  • c Nuffield Department of Clinical Medicine, Oxford Centre for Diabetes, Endocrinology, and Metabolism, University of Oxford, Oxford OX3 7LJ, United Kingdom, NIHR Oxford Biomedical Research Centre, Oxford Radcliffe Hospitals Trust, Oxford OX3 9DU, United Kingdom
  • d Nuffield Department of Clinical Medicine, Oxford Centre for Diabetes, Endocrinology, and Metabolism, University of Oxford, Oxford OX3 7LJ, United Kingdom
  • e Nuffield Department of Clinical Medicine, Oxford Centre for Diabetes, Endocrinology, and Metabolism, University of Oxford, Oxford OX3 7LJ, United Kingdom
  • f Nuffield Department of Clinical Medicine, Oxford Centre for Diabetes, Endocrinology, and Metabolism, University of Oxford, Oxford OX3 7LJ, United Kingdom
  • g Nuffield Department of Clinical Medicine, Oxford Centre for Diabetes, Endocrinology, and Metabolism, University of Oxford, Oxford OX3 7LJ, United Kingdom
  • h Nuffield Department of Clinical Medicine, Oxford Centre for Diabetes, Endocrinology, and Metabolism, University of Oxford, Oxford OX3 7LJ, United Kingdom, NIHR Oxford Biomedical Research Centre, Oxford Radcliffe Hospitals Trust, Oxford OX3 9DU, United Kingdom, Nuffield Department of Clinical Medicine, Oxford Centre for Diabetes, Endocrinology, and Metabolism, Churchill Hospital, Oxford OX3 7LJ, United Kingdom

Abstract

Context: Absence or excess of adipose tissue are both associated with metabolic complications, implying the importance of well-functioning adipose tissue present in normal amounts. Adipose tissue sequesters dietary fat and thus protects other tissues from excess fat exposure, especially after meals. Objective: The objective of the study was the use of an integrative physiological technique to quantify trafficking of fatty acids (FAs) in adipose tissue over a 24 h period. Methods: Adipose tissue FA handling was studied in response to three meals in eight healthy men by the combination of arteriovenous blood sampling, tissue blood flow, and specific labeling of FA tracing of exogenous and endogenous fat by stable isotope methodology. Results: The efficiency of adipose tissue FA uptake increased robustly with each meal. Chylomicron-triglyceride was the dominating source of FA. Adipose tissue fractional extraction of chylomicron-triglyceride increased from 21 ± 4 to 47 ± 8% (P = 0.03) between the first and last meal. Although adipose tissue lipoprotein lipase action increased with time (2-fold), there was an even greater increase in FA reesterification (3-fold), which led to a reduced spillover of chylomicron-derived FA, from 77 ± 15 to 34 ± 7% (P = 0.04) comparing the end of the first and the third meal period. Increased uptake of very low-density lipoprotein-derived FA was observed, but spillover of very low-density lipoprotein-derived FA was seen only in the fasting state. Conclusion: Human adipose tissue has a significant potential to up-regulate fat storage during a normal day that goes beyond increased lipoprotein lipase activation. The adaptation toward increasing fat storage may provide an explanation for the beneficial properties of normal amounts of adipose tissue. Copyright © 2009 by The Endocrine Society.

Author Keywords

[No Keywords available]

Index Keywords

blood sampling Adipose Tissue Blood Glucose Fasting esterification Cholesterol, VLDL Fatty Acids, Nonesterified oleic acid Regional Blood Flow physiology metabolism Up-Regulation upregulation vascularization palmitic acid derivative carbon 13 blood flow chylomicron Muscle, Skeletal human skeletal muscle very low density lipoprotein middle aged Chylomicrons lipoprotein lipase controlled study priority journal diet diet restriction linoleic acid very low density lipoprotein cholesterol lipid storage Triglycerides fatty acid blood level lipid blood level Fatty Acids Young Adult insulin fatty acid Humans male Lipid Metabolism Article blood glucose blood level adult human experiment Dietary Fats fat intake stable isotope triacylglycerol glucose normal human quantitative analysis body mass Body Mass Index low density lipoprotein forearm
Link
https://www.scopus.com/inward/record.uri?eid=2-s2.0-66149116077&doi=10.1210%2fjc.2008-2090&partnerID=40&md5=a339d71f400830e4d6b28b1d5c560037
DOI: 10.1210/jc.2008-2090
ISSN: 0021972X
Cited by: 91
Original Language: English