American Journal of Tropical Medicine and Hygiene
Volume 95, Issue 6, 2016, Pages 1330-1337

Multiplex polymerase chain reaction for detection of gastrointestinal pathogens in migrant workers in Qatar (Article) (Open Access)

Humphrey J.M.* , Ranbhise S. , Ibrahim E. , Al-Romaihi H.E. , Farag E. , Abu-Raddad L.J. , Glesby M.J.
  • a Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, 1305 York Avenue, New York, NY 10021, United States, Infectious Disease Epidemiology Group, Weill Cornell Medicine, Doha, Qatar
  • b Qatar Red Crescent Worker's Health Center, Doha, Qatar
  • c Hamad Medical Corporation, Doha, Qatar
  • d Communicable Diseases Department, Ministry of Public Health, Doha, Qatar
  • e Communicable Diseases Department, Ministry of Public Health, Doha, Qatar
  • f Infectious Disease Epidemiology Group, Weill Cornell Medicine, Doha, Qatar, Department of Healthcare Policy and Research, Weill Cornell Medicine, Cornell University, New York, NY, United States
  • g Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, 1305 York Avenue, New York, NY 10021, United States, Department of Healthcare Policy and Research, Weill Cornell Medicine, Cornell University, New York, NY, United States

Abstract

The causes of infectious diarrhea among the migrant worker population in Qatar are not well understood. We conducted a prospective observational study to understand the demographic and clinical characteristics and infectious causes of diarrhea among migrant workers in Doha, Qatar. A total of 126 male workers presenting to the Qatar Red Crescent Worker's Health Center outpatient clinic or emergency department were studied over a 5-month period in 2015-2016. Epidemiologic surveys were administered to all subjects and the prevalence of 22 different stool pathogens was determined using multiplex polymerase chain reaction (PCR) (FilmArray Gastrointestinal PCR). A target pathogen was identified in 62.7% of subjects. Enteropathogenic Escherichia coli was the most prevalent pathogen and was detected in 24.6% of subjects, followed by Salmonella (22.2%), enteroaggregative E. coli (15.1%), Giardia lamblia (9.5%), and enterotoxigenic E. coli (8.7%). Multiple pathogens were identified in 49.3% of positive stool samples. In a multivariable analysis, the presence of a heart rate ? 90 (adjusted odds ratio [OR] = 3.7, 95% confidence interval [CI] = 1.4-10.0) and > 5 fecal leukocytes/high-power field (adjusted OR = 2.8, 95% CI = 1.2-7.0) were significant predictors of detecting an acute inflammatory pathogen by PCR. Use of multiplex PCR enabled the detection of gastrointestinal pathogens in a high proportion of cases, illustrating the utility of this diagnostic tool in epidemiologic studies of infectious diarrhea. Copyright © 2016 by The American Society of Tropical Medicine and Hygiene.

Author Keywords

[No Keywords available]

Index Keywords

parasitology Giardia intestinalis prospective study Multiplex Polymerase Chain Reaction Escherichia coli ciprofloxacin Heart Rate human Qatar Salmonella Shigella Adenoviridae enterotoxigenic Escherichia coli Astroviridae enteropathogenic Escherichia coli infectious diarrhea Norovirus enteroaggregative Escherichia coli enteroinvasive Escherichia coli nonhuman emergency ward polymerase chain reaction system Plesiomonas shigelloides Rotavirus A procedures migrant worker Humans virology male female Virus Diseases Cyclospora cayetanensis Campylobacter observational study outpatient department polymerase chain reaction Coinfection Article cotrimoxazole major clinical study adult Bacterial Infections antibiotic therapy migration microbiology antiinfective agent Transients and Migrants Anti-Bacterial Agents microorganism detection metronidazole Gastrointestinal Diseases mixed infection Parasitic Diseases
Link
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85005976141&doi=10.4269%2fajtmh.16-0464&partnerID=40&md5=ade7842c56f7c8934b558092a6696211
DOI: 10.4269/ajtmh.16-0464
ISSN: 00029637
Cited by: 3
Original Language: English